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Identification of avermectin-high-producing strains by high-throughput screening methods.

Hong Gao1, Mei Liu, Xianlong Zhou

  • 1Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, People's Republic of China.

Applied Microbiology and Biotechnology
|December 4, 2009
PubMed
Summary

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This study developed a rapid, miniaturized screening method to identify high-yield avermectin-producing strains. This efficient process accelerates the discovery of superior strains for avermectin production.

Area of Science:

  • Microbiology
  • Biotechnology
  • Parasitology

Background:

  • Avermectins are potent antiparasitic agents produced by *Streptomyces avermitilis*.
  • Efficient identification of high-yield strains is crucial for avermectin production.
  • Current screening methods can be time-consuming and resource-intensive.

Purpose of the Study:

  • To develop and validate a high-throughput screening strategy for avermectin production.
  • To miniaturize the avermectin production and monitoring protocol.
  • To accelerate the selection of superior avermectin-producing strains.

Main Methods:

  • Miniaturization of avermectin production in 96 deep-well microplates.
  • UV absorbance at 245 nm for rapid monitoring of avermectin production.

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  • Correlation analysis between microplate and conventional flask fermentation.

    • Main Results:

    • A robust correlation was observed between microplate and flask fermentation results.
    • Avermectin production was accurately determined in under 10 minutes per plate.
    • The selected high-yield strain showed a 1.6-fold improvement in large-scale fermentation.

    Conclusions:

    • The developed high-throughput screening protocol is efficient and accurate.
    • This method significantly accelerates the selection of avermectin-producing strains.
    • The protocol is expected to enhance the overall efficiency of avermectin production.