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Published on: December 16, 2017
Live cell imaging: an industrial perspective
1TJM Consultancy, Kent, UK.
Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2009
Summary
High content screening/analysis (HCS/A) enables large-scale live cell analysis for drug discovery and systems cell biology. This technology requires careful consideration of cells, probes, and assays, with various instrumentation options available.
Area of Science:
- Cell biology
- Pharmacology
- Biotechnology
Background:
- High content screening/analysis (HCS/A) is automated fluorescence microscopy for industrial-scale live cell analysis.
- Driven by drug discovery compound screening and functional genomics (siRNA knockouts).
Purpose of the Study:
- Outline HCS/A applications in drug discovery and systems cell biology.
- Discuss critical considerations for HCS/A implementation.
- Detail user requirements and available instrumentation for live cell HCS/A.
Main Methods:
- Literature review and synthesis of HCS/A applications.
- Analysis of user requirements through industry and academic surveys.
- Overview of current live cell HCS/A imaging instrumentation.
Main Results:
- Identified key applications of HCS/A in pharmaceutical research and cell biology.
- Highlighted essential factors for successful HCS/A: cell choice, probes, labels, and assay design.
- Summarized user needs and the landscape of available imaging technologies.
Conclusions:
- HCS/A is a vital tool for modern biological research and drug development.
- Optimizing HCS/A involves careful planning of experimental parameters and technology selection.
- The field continues to evolve with advancements in instrumentation and applications.
