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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

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Published on: August 17, 2015

Methods and algorithms for relative quantitative proteomics by mass spectrometry.

Rune Matthiesen1, Ana Sofia Carvalho

  • 1Instituto de Patologia e Imunologia Molecular da Universidad do Porto - IPATIMUP, Porto, Portugal.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2009
PubMed
Summary
This summary is machine-generated.

Mass spectrometry (MS) offers protein identification and quantification, unlike protein arrays. This overview covers common MS quantitative methods and computational algorithms for robust proteomics analysis.

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Mass spectrometry (MS) enables simultaneous protein identification and quantification, including post-translational modifications.
  • Protein arrays are limited to quantifying targeted proteins and their modifications.
  • Numerous quantitative MS methods exist for proteins and peptides.

Purpose of the Study:

  • To provide an overview of common MS-based quantitative methods in proteomics.
  • To discuss computational algorithms essential for accurate protein and peptide quantification.
  • To cover the robust quantitation of post-translational modifications using MS.

Main Methods:

  • Review of established quantitative mass spectrometry techniques.
  • Discussion of computational algorithms for data analysis.
  • Focus on methods for protein, peptide, and PTM quantitation.

Main Results:

  • Identification of prevalent MS-based quantitative strategies.
  • Highlighting the importance of computational tools for reliable results.
  • Comparison of different approaches for protein and PTM analysis.

Conclusions:

  • MS is a powerful tool for comprehensive protein quantification.
  • Appropriate method selection and computational analysis are crucial for robust proteomics.
  • This overview aids researchers in choosing and applying quantitative MS techniques.