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[Using protein chips to explore the differential expression of serum proteins in healthy women and OEC patients]
Fei Chen1, Jia-xin Yang, Ming Wu
1Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing.
Objective:
To explore the differentially expressed serum proteins in healthy women and OEC patients by using protein chips.
Methods:
Sera of 11 cases of EOC and 11 matched healthy women were labeled with cy3 and cy5 fluorescent dyes and then were made to hybridized with the same protein antibody chips. The internally normalized ratio (INR) was calculated according to the fluorescent intensity of each protein spot. The value of INR > 2.0 or < 0.7 was considered as a cut-value to screen the differentially expressed serum proteins in EOC patients and healthy women.
Results:
Twenty-seven differentially expressed serum proteins were found in healthy women and EOC patients, including 17 up-regulated and 10 down-regulated proteins in EOC patients.
Conclusion:
Proteome-protein antibody chip can be used to detect the differentially expressed serum proteins in the tissues. These target proteins will facilitate the study of diagnostic biomarkers of EOC and aid in monitoring the therapeutic effect of EOC.
