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Updated: Jun 18, 2026

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
[Expression of recombinant cytolethal distending toxin of Actinobacillus actinomycetemcomitans]
1State Key Laboratory of Oral Disease, Sichuan University, Chengdu 610041, China.
Objective:
To examine the expression of recombinant cytolethal distending toxin (CDT) produced by Actinobacillus actinomycetemcomitans (Aa).
Methods:
CDT encoding gene cdtABC was amplified by PCR. Through TA clone and restriction endonuclease digestion, gene cdtABC and vector pQE60 were ligated to form pQE60-cdtABC expression system which transformed into competent cells. Protein expression was induced by IPTG and examined by SDS-PAGE and Western-blotting.
Results:
Random colony PCR of pQE60-cdtABC transformed cells demonstrated that all strains contained cdtABC gene. The DNA sequence was blast with cdtABC gene from GenBank and 99% homology was obtained. SDS-PAGE and Western-blotting confirmed that recombinant CDT was obtained.
Conclusions:
CDT protein expression system was reconstructed and recombinant protein was obtained. Actinobacillus actinomycetemcomitans;
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