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Updated: Jun 18, 2026
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An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis
Published on: August 6, 2020
Robo1: a potential role in ocular angiogenesis.
Lvzhen Huang1, Yongsheng Xu, Wenzhen Yu
1Department of Ophthalmology, Peking University People's Hospital, Xizhimen South Street 11, Xi Cheng District, Beijing, China.
Current Eye Research
|December 5, 2009
Summary
Robo1, a neuronal guidance receptor, was found to inhibit retinal angiogenesis. This suggests Robo1 may be a therapeutic target for conditions involving abnormal blood vessel growth in the eye.
Area of Science:
- Ophthalmology
- Molecular Biology
- Cell Biology
Background:
- Robo1 (roundabout guidance receptor 1) is known to mediate tumor angiogenesis.
- Its role in retinal and choroidal angiogenesis is not well understood.
Purpose of the Study:
- To investigate the function of Robo1 in retinal angiogenesis.
- To explore Robo1's potential as a therapeutic target for angiogenesis-related eye diseases.
Main Methods:
- Robo1 expression was analyzed in a mouse model of retinopathy of prematurity (ROP).
- Robo1 was knocked down using siRNA in monkey choroidal retinal endothelial cells (RF/6A) in vitro.
- Cell proliferation, migration, apoptosis, and tube formation assays were performed.
Main Results:
- Robo1 expression was significantly elevated in ROP mouse eyes.
- Robo1 knockdown inhibited RF/6A cell proliferation and migration.
- Tube formation by RF/6A cells was impaired following Robo1 knockdown.
Conclusions:
- Robo1 inhibits choroidal and retinal angiogenesis in vitro.
- Robo1 represents a potential therapeutic target for treating choroidal or retinal angiogenesis.
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