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Published on: February 15, 2020
Oxidative macromolecular damage in age-related macular degeneration
Yüksel Totan1, Ramazan Yağci, Yavuz Bardak
1Fatih University, Medical School, Department of Ophthalmology, Ankara, Turkey.
Purpose:
To evaluate oxidative damage to the macromolecules, including protein, lipid, and DNA, in association with total oxidation status (TOS), total antioxidant capacity (TAC) in patients with exudative age-related macular degeneration (AMD).
Materials And Methods:
Serum levels of malondialdehyde (MDA) as an index of lipid peroxidation, protein carbonyl (PC) as a marker of protein oxidation, 8-hydroxy-29-deoxyguanosine (8-OHdG) as an indicator of oxidative DNA damage along with TOS, TAC were measured by specific methods in 47 patients with exudative AMD and 25 age- and sex-matched healthy subjects.
Results:
Significantly higher MDA, PC, 8-OHdG, TOS, and lower TAC levels were detected in the serum of patients with exudative AMD compared with their controls (p < 0.001).
Conclusion:
The results indicate that an imbalance between TOS and TAC leads to not only increased lipid damage, but also protein and DNA damage. These first reported results suggest that protein and DNA damage might also play a role in the pathogenesis of AMD.
Insights
Oxidative damage to lipids, proteins, and DNA is elevated in exudative age-related macular degeneration (AMD). This imbalance in total oxidation status (TOS) and total antioxidant capacity (TAC) suggests a role in AMD pathogenesis.
Area of Science:
- Ophthalmology
- Biochemistry
- Molecular Biology
Background:
- Age-related macular degeneration (AMD) is a leading cause of vision loss.
- Oxidative stress is implicated in the pathogenesis of various diseases, including AMD.
Purpose of the Study:
- To evaluate oxidative damage to macromolecules (protein, lipid, DNA) in exudative AMD.
- To assess the association between total oxidation status (TOS) and total antioxidant capacity (TAC) in AMD patients.
Main Methods:
- Serum levels of malondialdehyde (MDA), protein carbonyl (PC), and 8-hydroxy-29-deoxyguanosine (8-OHdG) were measured.
- TOS and TAC levels were determined in 47 exudative AMD patients and 25 healthy controls.
Main Results:
- Patients with exudative AMD showed significantly higher MDA, PC, 8-OHdG, and TOS levels.
- Lower TAC levels were observed in the serum of exudative AMD patients compared to controls (p < 0.001).
Conclusions:
- An imbalance between TOS and TAC contributes to increased lipid, protein, and DNA damage in AMD.
- Protein and DNA damage may play a significant role in the pathogenesis of exudative AMD.
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