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Related Concept Videos

Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...

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JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
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JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

MitoInteractome: mitochondrial protein interactome database, and its application in 'aging network' analysis.

Rohit Reja1, A J Venkatakrishnan, Jungwoo Lee

  • 1Korean Bioinformation Center, KRIBB, Daejeon, 305-806, Korea. rohit@kribb.re.kr

BMC Genomics
|December 5, 2009
PubMed
Summary

MitoInteractome is a new database detailing mitochondrial proteome interactions, properties, and diseases. This resource aids understanding of mitochondrial functions and identifies potential drug targets for aging and other conditions.

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JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
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Optimized Automated Analysis of Live Neuronal Mitochondria Homeostasis Modulation by Isoform-Specific Retinoic Acid Receptors

Published on: July 28, 2023

Area of Science:

  • Mitochondrial biology
  • Proteomics
  • Bioinformatics

Background:

  • Mitochondria are crucial for cellular energy production and apoptosis.
  • Mitochondrial proteins are encoded by both nuclear and mitochondrial genes.
  • An expanding number of mitochondrial sequences necessitates a centralized database.

Purpose of the Study:

  • To develop a consolidated web-based portal for mitochondrial proteome information.
  • To provide data on protein-protein interactions, physico-chemical properties, polymorphism, and diseases.
  • To facilitate research into mitochondrial functions and disease mechanisms.

Main Methods:

  • Consolidated data from SwissProt, MitoP, MitoProteome, HPRD, and Gene Ontology.
  • Construction of the mitochondrial interactome using PSIMAP and PEIMAP based on 'homologous interaction'.
  • Prediction of protein-protein interactions for 74 species.

Main Results:

  • MitoInteractome database contains 6,549 protein sequences.
  • Predicted protein-protein interactions for 74 species, including a human aging network.
  • Analysis of the aging network provided insights into protein interactions influencing aging.

Conclusions:

  • MitoInteractome enhances understanding of mitochondrial protein functions and networks.
  • Facilitates identification of novel protein targets for research and drug development.
  • Aids in discovering diagnostic biomarkers and therapeutic targets related to mitochondrial diseases.