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Application of DNA Barcoding to Identify Medicinal Plants
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BioBarcode: a general DNA barcoding database and server platform for Asian biodiversity resources.

Jeongheui Lim1, Sang-Yoon Kim, Sungmin Kim

  • 1Korean BioInformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea. jhlim@kribb.re.kr

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|December 5, 2009
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A new BioBarcode system offers a standardized, open-source solution for DNA barcoding data processing. This tool aids in mapping Asia

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Area of Science:

  • Bioinformatics
  • Genomics
  • Biodiversity Science

Background:

  • DNA barcoding enables rapid, standardized species identification using short DNA sequences.
  • Accurate species mapping is crucial for global biodiversity assessment, especially with decreasing DNA sequencing costs.
  • Asia possesses rich biodiversity requiring robust systems for mapping and database registration.

Purpose of the Study:

  • To develop a general, open-source DNA barcode data processing system for Asian researchers.
  • To facilitate efficient bioinformatics protocols for DNA barcoding in biodiversity studies.
  • To promote the rapid acquisition and analysis of standardized biological species DNA sequence data.

Main Methods:

  • Development of the BioBarcode system using open-source software (mySQL RDBMS 5.0, BLAST2, Apache httpd server).
  • Creation of a general-purpose database and server for DNA barcode data.
  • Integration of a chromatogram viewer for enhanced DNA sequence analysis.

Main Results:

  • A functional BioBarcode system and database were established.
  • The exemplary database contains approximately 11,300 specimen entries, including GenBank data.
  • The system facilitates mapping genetic relationships among biological species.

Conclusions:

  • The BioBarcode system provides an efficient, freely accessible bioinformatics protocol for Asian biodiversity research.
  • It standardizes, deposits, manages, and analyzes DNA barcode data, making barcoding cheaper and faster.
  • An exemplary server (http://www.asianbarcode.org) is available for building an Asian biodiversity system.