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Updated: Jun 18, 2026

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Published on: July 7, 2010

[LRP16 gene function based on bioinformatic analysis].

Bo Yang¹, Xue-Chun Lu, Xiao-Hua Chi

¹Department of Geriatric Hematology, PLA General Hospital, Beijing 100853, P. R. China.

AI Zheng = Aizheng = Chinese Journal of Cancer

|December 5, 2009

Summary

This summary is machine-generated.

The novel human gene LRP16 promotes leukemia progression by enhancing cell proliferation and regulating the cell cycle. LRP16 also helps protect against radiation-induced DNA damage.

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Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

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RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

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Published on: May 19, 2019

Area of Science:

Molecular Biology
Genetics
Bioinformatics

Background:

Leukemia is a complex disease involving genetic alterations.
LRP16 is a recently identified human gene associated with leukemia.
The precise biological functions of LRP16 remain largely uncharacterized.

Purpose of the Study:

To elucidate the biological functions of the human LRP16 gene.
To analyze the regulatory elements of the LRP16 gene promoter.
To investigate the role of LRP16 in cellular processes relevant to cancer.

Main Methods:

Bioinformatics analysis of LRP16 gene promoter structure and function.
Luciferase reporter assays to assess promoter activity.
Construction and transfection of LRP16 expression vectors into leukemia cell lines (HL-60 and K562).
Evaluation of DNA damage repair and cell cycle progression using comet assays and flow cytometry.

Main Results:

LRP16 promoter identified as a Class II eukaryotic promoter with key regulatory elements within -600 bp.
Seven cis-acting elements potentially involved in cell cycle, hematopoiesis, proliferation, and DNA damage repair were identified.
Overexpression of LRP16 reduced DNA damage and increased cell viability in irradiated HL-60 cells.
LRP16 overexpression in K562 cells enhanced proliferation, promoted G1 to S phase transition, and advanced the cell proliferation plateau.

Conclusions:

Bioinformatics-driven promoter and protein domain analysis is valuable for gene function studies.
LRP16 plays a significant role in leukemia progression.
LRP16 promotes cell proliferation, regulates cell cycle, and confers resistance to radiation-induced DNA damage.