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Related Concept Videos

Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

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Preparation of Monodomain Liquid Crystal Elastomers and Liquid Crystal Elastomer Nanocomposites
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Poly(2-ethyl-2-oxazoline) as a sieving matrix for SDS-CE.

Richard Bernard1, Gary Loge

  • 1LCM Technologies, Inc., State College, PA 16803, USA.

Electrophoresis
|December 5, 2009
PubMed
Summary

Poly(2-ethyl-2-oxazoline) at 10% w/v is an effective sieving matrix for sodium dodecyl sulfate capillary electrophoresis (SDS-CE) protein separation. It offers high separation efficiency and stability for laser-induced fluorescence detection.

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Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry

Published on: June 10, 2018

Area of Science:

  • Analytical Chemistry
  • Polymer Science
  • Biochemistry

Background:

  • Capillary electrophoresis (CE) is a powerful separation technique.
  • Sodium dodecyl sulfate (SDS) is commonly used for protein separation based on size.
  • Developing efficient sieving matrices is crucial for enhancing SDS-CE performance.

Purpose of the Study:

  • To investigate the efficacy of poly(2-ethyl-2-oxazoline) as a sieving matrix for SDS-CE.
  • To optimize polymer concentration for maximum separation efficiency.
  • To evaluate buffer systems and wall coatings for improved performance.

Main Methods:

  • SDS-Capillary Electrophoresis (SDS-CE) was employed.
  • Poly(2-ethyl-2-oxazoline) solutions of varying concentrations (6-12% w/v) were tested.
  • Two buffer systems (HEPES and Tris-tricene) and wall coatings (adsorbed polymer and polyacrylamide) were evaluated.
  • Laser-induced fluorescence (LIF) detection was utilized.

Main Results:

  • Optimal separation efficiency was achieved with 10% w/v poly(2-ethyl-2-oxazoline).
  • High theoretical plate counts were observed for myoglobin and carbonic anhydrase.
  • Tris-tricene buffer offered better current stability than HEPES.
  • Covalently attached polyacrylamide coating proved less troublesome than adsorbed polymer.

Conclusions:

  • Poly(2-ethyl-2-oxazoline) is a promising sieving matrix for SDS-CE protein separations.
  • The polymer exhibits desirable characteristics such as hydrolytic stability, optical clarity, and low viscosity.
  • Further optimization of buffer and coating systems can enhance SDS-CE performance.