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[Effect of radiation on the activity and expression of Ca(2+)-Mg(2+)-ATPase in rat masseter muscle]
Zhi-Min Li1, Xu-Chen Ma, Xing-Min Qu
1Department of Oral Radiology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Objective:
To investigate the activity and expression of Ca(2+)-Mg(2+)-ATPase in irradiated rat masseter muscle.
Methods:
The rats were irradiated locally with a single dose of 20 Gy X-ray. The activities of Ca(2+)-Mg(2+)-ATPase were measured with colorimetric method. The protein expression of Ca(2+)-Mg(2+)-ATPase was determined by Western blotting and immunohistochemistry.
Results:
The activities of Ca(2+)-Mg(2+)-ATPase in masseter muscle decreased by approximately 20% and 40% in irradiated rats on days 3 and 30 postirradiation. There was significant difference in the expression of Ca(2+)-Mg(2+)-ATPase protein between irradiated and nonirradiated rats on day 30 postirradiation. Ca(2+)-Mg(2+)-ATPase protein was found in the cytoplasm of masseter muscle.
Conclusions:
The decrease of ATPase activity played an important role in the cause of radiation-induced skeletal muscle injury, while there was no significant reduction in the expression of Ca(2+)-Mg(2+)-ATPase protein in irradiated rat masseter muscle.
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