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Related Concept Videos

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Published on: December 9, 2022

5S rRNA-assisted DnaK refolding.

Hyo Kyung Kim1, Seong Il Choi, Baik L Seong

  • 1Department of Biotechnology, College of Bioscience and Biotechnology, Yonsei University, Seoul 120-749, South Korea.

Biochemical and Biophysical Research Communications
|December 8, 2009
PubMed
Summary
This summary is machine-generated.

This study shows that 5S ribosomal RNA (rRNA) can assist in the in vitro refolding of the molecular chaperone DnaK. This suggests RNA molecules may play a chaperoning role for interacting proteins.

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Published on: July 9, 2021

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • While protein folding often occurs independently, the role of other macromolecules in this process is less understood.
  • Many proteins interact with RNA in vivo, hinting at a potential functional relationship.
  • The chaperoning activity of macromolecules beyond proteins remains an under-explored area.

Purpose of the Study:

  • To investigate the potential role of RNA in assisting the folding of its interacting proteins.
  • To determine if 5S ribosomal RNA (rRNA) can facilitate the refolding of the molecular chaperone DnaK.
  • To explore the mechanism and specificity of RNA-mediated protein folding assistance.

Main Methods:

  • In vitro refolding assays were performed on DnaK, a bacterial Hsp70 homolog.
  • The effect of 5S rRNA on DnaK refolding was assessed at varying RNA concentrations and sizes.
  • Control experiments included using a reverse-sequence 5S rRNA and RNase-treated 5S rRNA.

Main Results:

  • 5S rRNA significantly enhanced the in vitro refolding of DnaK.
  • The folding enhancement was dependent on both RNA concentration and size.
  • Neither a scrambled sequence RNA nor degraded RNA showed a similar effect, indicating specificity.

Conclusions:

  • 5S rRNA exhibits chaperoning activity, assisting the folding of DnaK.
  • This finding suggests that cognate RNA ligands may play a role in the folding of RNA-interacting proteins.
  • The study opens new avenues for understanding non-proteinaceous chaperoning mechanisms in cellular processes.