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Updated: Jun 18, 2026

Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays
Published on: February 12, 2022
UV-vis, IR and 1H NMR spectroscopic studies and characterization of ionic-pair crystal violet-oxytetracycline
Sandra Orellana1, César Soto, M Inés Toral
1Department of Chemistry, Faculty of Sciences, University of Chile, Santiago, Chile. analitic@uchile.cl
Abstract:
The present study shows the formation and characterization of the ionic-pair between the antibiotic oxytetracycline and the dye crystal violet in ammonia solution pH 9.0+/-0.2 extracted into chloroform. The characterization was demonstrated using UV-vis spectrophotometry, (1)H NMR, measurement of relaxation times T(1) and IR spectroscopy, using a comparison between the signals of individual pure compounds with the signals with the mixture CV-OTC in different alkaline media. The formation of ionic-pair was also corroborated by new signals and chemical shifts. (2D) NMR spectroscopy experiments show that the interaction is electrostatic.
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