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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

The Movement of Organelles and Vesicles

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In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...

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Related Experiment Video

Updated: Jun 18, 2026

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Published on: August 16, 2021

Motion detection for subcellular structure trafficking.

Hidekazu Iwaki¹, Akio Kosaka, Shuo Li

¹Future Creation Laboratory Olympus Corporation Tokyo, Japan.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference

|December 8, 2009

Summary

This summary is machine-generated.

Understanding subcellular dynamics is key to cell function. We developed a new "divergence filter" technique to accurately detect various subcellular structure motion patterns, improving cell tracking and analysis.

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Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Area of Science:

Cell Biology
Biophysics
Microscopy

Background:

Subcellular structure motility is crucial for protein transport and cellular regulation.
Diverse motion patterns (directional, random, tethered, appearance/disappearance) exist.
Accurate detection of these motion modalities is vital for cell tracking and population studies.

Purpose of the Study:

To introduce a novel technique for detecting subcellular structure motion modalities.
To enhance the efficiency of subcellular structure tracking and population studies.
To validate the technique using real-world biological data.

Main Methods:

Development of a new computational technique named "divergence filter".
Application of the divergence filter to analyze subcellular dynamics.
Utilizing confocal microscopy image sequences of caveolar membrane trafficking.

Main Results:

The divergence filter effectively detects diverse subcellular motion modalities.
The technique demonstrates efficiency in analyzing complex cellular dynamics.
Successful application to caveolar membrane trafficking sequences.

Conclusions:

The divergence filter is a plausible and efficient method for identifying subcellular motion patterns.
This technique advances the study of subcellular dynamics and protein transport.
Improved motion modality detection facilitates deeper understanding of cellular processes.