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Updated: Jun 18, 2026

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Fluorescence biosensing in nanopores.

Jan Karolin1, Dalibor Pánek, Alexander MacMillan

  • 1Department of Physics, University of Strathclyde, SUPA, G4 0NG, Glasgow, Scotland, UK. jan.karolin@strath.ac.uk

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|December 8, 2009
PubMed
Summary
This summary is machine-generated.

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Researchers developed biocompatible nanopores for studying single molecules and creating sensors. Using PRODAN (6-propionyl-2-(N,N-dimethylamino) naphthalene) fluorescence, they preserved proteins like allophycocyanin (APC) in silica and alumina nanopores for extended periods.

Area of Science:

  • Nanotechnology
  • Biophysics
  • Materials Science

Background:

  • Hydrated nanopores provide controlled environments for studying biological molecules and fabricating fluorescent sensors.
  • Silica nanopores are non-toxic, protein-compatible, and can be synthesized to entrap proteins while allowing small analytes to pass.
  • Alcohol release during sol-gel nanopore fabrication denatures proteins, posing a challenge for biocompatibility.

Purpose of the Study:

  • To develop a biocompatible protocol for fabricating sol-gel nanopores using fluorescence monitoring.
  • To demonstrate the preservation of unstable protein structures within these improved nanopores.
  • To extend protein encapsulation techniques to different nanopore materials like alumina.

Main Methods:

  • Utilized PRODAN (6-propionyl-2-(N,N-dimethylamino) naphthalene) fluorescence to monitor methanol release during orthosilicate polymerization.

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Optical Trapping of Nanoparticles
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  • Synthesized silica sol-gel nanopores using tetramethyl orthosilicate (TMOS) and assessed protein preservation.
  • Employed PRODAN and Nile red (NR) dyes to characterize pore polarity and extended encapsulation to alumina nano-channels.

    • Main Results:

    • Developed a protocol with enhanced biocompatibility by monitoring methanol using PRODAN fluorescence.
    • Successfully preserved the native trimer form of allophycocyanin (APC) in silica nanopores for up to 500 hours.
    • Demonstrated single-molecule observation of native APC trimers via fluorescence within nanopores.
    • Extended protein encapsulation to alumina nano-channels, reporting on pore polarity with PRODAN and Nile red.

    Conclusions:

    • Improved sol-gel nanopore fabrication enhances biocompatibility, enabling long-term preservation of sensitive protein structures.
    • This advancement allows for single-molecule studies of proteins like allophycocyanin (APC) in their native states within nanopores.
    • The developed methods are applicable to nanomedicine for disease pathology research and the creation of advanced biosensors for detecting non-fluorescent metabolites.