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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Jun 18, 2026

Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation
08:58

Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation

Published on: July 31, 2013

Pulse-clamp technique for single neuron stimulation electrode characterization.

Andre van Ooyen1, Volker G Zagolla, Christian Ulrich

  • 1Institute of Materials in Electrical Engineering I, RWTH Aachen University, 52074 Aachen, Germany. vanooyen@iwe1.rwth-aachen.de

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|December 8, 2009
PubMed
Summary

A new pulse-clamp circuit effectively evaluates microelectrode charge injection for neural stimulation. Sputtered iridium oxide film (SIROF) electrodes show superior performance over platinum, enabling high-resolution neural interfaces.

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Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
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Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Published on: January 10, 2015

Area of Science:

  • Neuroscience
  • Materials Science
  • Electrical Engineering

Background:

  • Miniaturized electrodes are crucial for selective neural stimulation.
  • High charge injection is needed for effective neural stimulation.
  • A reliable method to test charge injection in small electrodes is required.

Purpose of the Study:

  • To develop and utilize a pulse-clamp circuit for characterizing microelectrode charge injection.
  • To compare the charge injection capabilities of different microelectrode materials and sizes.
  • To assess the suitability of microelectrodes for high-resolution neural stimulation.

Main Methods:

  • A pulse-clamp circuit was designed and employed to measure charge storage.
  • Planar microelectrodes (<300 microm diameter) were tested in phosphate-buffered saline (PBS).
  • Cyclic voltammetry was used alongside pulse-clamp measurements for comparison.

Main Results:

  • The pulse-clamp circuit allows rapid and accurate comparison of electrode performance.
  • Sputtered iridium oxide film (SIROF) electrodes demonstrated <3% charge loss.
  • SIROF electrodes exhibited superior reversible charge injection compared to platinum electrodes at high charge densities.

Conclusions:

  • The developed pulse-clamp technique is effective for evaluating microelectrode charge injection capabilities.
  • SIROF microelectrodes offer significant advantages for neural stimulation applications requiring high charge density.
  • This work facilitates the development of advanced neural interfaces for precise stimulation.