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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: Jun 18, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Differential gene expression using mRNA isolated on plastic microfluidic chips.

Arpita Bhattacharyya1, Catherine M Klapperich

  • 1Department of Biomedical Engineering, Boston University, Boston, MA, USA. arpitabhattacharyya@gmail.com

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|December 8, 2009
PubMed
Summary
This summary is machine-generated.

This study presents a novel microfluidic device for isolating messenger RNA (mRNA) directly from cell lysates. This method enables efficient differential gene expression analysis, simplifying molecular biology workflows.

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Microfluidics

Background:

  • Differential gene expression analysis is crucial for understanding cellular function and disease.
  • Traditional mRNA isolation methods can be time-consuming and require significant sample input.

Purpose of the Study:

  • To develop and validate a microfluidic solid-phase extraction system for direct messenger RNA (mRNA) isolation from crude cell lysates.
  • To demonstrate the utility of this system for differential gene expression experiments.

Main Methods:

  • Fabrication of plastic microfluidic columns using hot-embossing.
  • Development of a photopolymerized microporous monolith solid-phase embedded with oligo(dT) beads for mRNA capture.
  • Application of the microfluidic system for mRNA isolation from human liver total RNA, neonatal human dermal fibroblast (NHDF) cell lysates, and MCF7 breast cancer cell lysates.

Main Results:

  • Achieved an approximate 80% extraction efficiency for mRNA.
  • Demonstrated a nucleic acid binding capacity of approximately 3.5 ng for the silica solid-phase.
  • Successfully performed differential gene expression analysis between NHDF and MCF7 cell lines using the isolated mRNA.

Conclusions:

  • The developed microfluidic solid-phase extraction column provides an efficient method for direct mRNA isolation from cell lysates.
  • This technology simplifies the workflow for gene expression studies and has potential applications in various research settings.