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Related Experiment Video

Updated: Jun 18, 2026

Quantitative Autonomic Testing

Quantitative Autonomic Testing

Published on: July 19, 2011

Does sweat volume influence the sweat test result?

Shmuel Goldberg¹, Shepard Schwartz, Francis Mimouni

¹Department of Pediatric Pulmonology, Shaare Zedek Medical Center, Hebrew University Medical School, Jerusalem, Israel. sgoldberg@szmc.org.il

Archives of Disease in Childhood

|December 8, 2009

Summary

This summary is machine-generated.

Low sweat volume samples are not always unreliable for cystic fibrosis diagnosis. While a weak inverse relationship exists between sweat volume and conductivity, low volumes may yield slightly higher, potentially artefactual, results.

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Published on: February 10, 2016

Area of Science:

Clinical diagnostics
Biochemistry

Background:

Low sweat volume samples are traditionally considered unreliable for cystic fibrosis (CF) diagnosis.
This is based on the assumption that sweat conductivity and chloride levels decrease at lower sweating rates.

Purpose of the Study:

To re-evaluate the relationship between sweat volume and diagnostic test results for cystic fibrosis.
To determine the reliability of low-volume sweat samples in CF diagnosis.

Main Methods:

Retrospective review of all sweat tests performed at the institution.
Assessment of the relationship between sweat volume and sweat conductivity.
Assessment of the relationship between sweat volume and sweat chloride.
Comparison of results from simultaneously collected sweat samples with volumes above and below the 15 microl minimum.

Main Results:

A weak inverse relationship was observed between sweat volume and sweat conductivity (n=1500, R²=0.105, p<0.001).
No significant correlation was found between sweat volume and sweat chloride (n=463, R²=0.002, p>0.05).
In discordant pairs, lower volume samples (<15 microl) showed slightly higher mean conductivity (41.1 vs 36.8 mmol/l, p<0.001) and chloride (28.4 vs 25.1 mmol/l, p=0.004) compared to higher volume samples.

Conclusions:

Normal sweat conductivity or chloride from low-volume samples (<15 microl) makes cystic fibrosis unlikely in patients without strong clinical suspicion.
Elevated results from low-volume samples (<15 microl) may be artefactual and related to the low sample volume.