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Related Experiment Video

Updated: Jun 18, 2026

Isolation of Preadipocytes from Broiler Chick Embryos
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Isolation of Preadipocytes from Broiler Chick Embryos

Published on: August 4, 2022

Gluconeogenesis differs in developing chick embryos derived from small compared with typical size broiler breeder

N E Sunny1, B J Bequette

  • 1Department of Animal and Avian Sciences, University of Maryland, College Park 20742, USA.

Journal of Animal Science
|December 8, 2009
PubMed
Summary

Embryos from smaller eggs show higher rates of glucose production earlier in development. This may lead to reduced growth as amino acids are diverted to energy production.

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Area of Science:

  • Biochemistry
  • Developmental Biology
  • Animal Physiology

Background:

  • Embryonic development relies on glucose supply.
  • As glucose diminishes, embryos may shift to gluconeogenesis (GNG).
  • Egg size can influence nutrient availability and embryonic growth.

Purpose of the Study:

  • To investigate if broiler embryos transition to increased gluconeogenesis (GNG) as glucose supply decreases.
  • To compare GNG rates between embryos from small and typical-sized eggs.
  • To determine the impact of egg size on embryonic glucose metabolism and growth.

Main Methods:

  • Administered isotopically labeled glucose to broiler embryos from small and typical-sized eggs.
  • Measured blood glucose enrichment using gas chromatography-mass spectrometry at multiple embryonic days (e12-e18).

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Published on: September 20, 2021

Windowing Chicken Eggs for Developmental Studies
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Published on: October 1, 2007

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Last Updated: Jun 18, 2026

Isolation of Preadipocytes from Broiler Chick Embryos
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Published on: August 4, 2022

In Ovo Feeding of Commercial Broiler Eggs: An Accurate and Reproducible Method to Affect Muscle Development and Growth
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  • Quantified glucose entry rate, Cori cycle flux, and gluconeogenic amino acid concentrations.

    • Main Results:

    • Embryos from small eggs weighed less from e14 onwards.
    • Glucose metabolism rates (entry, Cori cycle) increased with development in both groups.
    • Embryos from small eggs exhibited higher glucose entry and flux rates, especially earlier in development.
    • Gluconeogenic amino acid concentrations were lower in embryos from small eggs.

    Conclusions:

    • Embryos from small eggs demonstrate higher gluconeogenesis rates earlier in development.
    • This increased reliance on GNG may contribute to reduced embryonic growth in smaller eggs.
    • Amino acid diversion to gluconeogenesis could be a key factor in growth limitation.