Optimization of xylanase production by Thermomyces lanuginosus in solid state fermentation

Area of Science:

• Biotechnology
• Enzyme Technology
• Microbial Fermentation

Background:

• Xylanases are crucial enzymes with diverse industrial applications.
• Thermophilic fungi offer advantages for high-temperature enzymatic processes.
• Solid-state fermentation (SSF) is an efficient method for microbial enzyme production.

Purpose of the Study:

• To optimize solid-state fermentation (SSF) parameters for enhanced extracellular xylanase production by Thermomyces lanuginosus 195.
• To investigate the impact of supplemental carbon and nitrogen sources on xylanase yield.
• To determine optimal conditions for maximum xylanase activity.

Main Methods:

• Solid-state fermentation (SSF) using Thermomyces lanuginosus 195 on wheat bran.
• Systematic optimization of fermentation temperature, duration, and inoculum volume.
• Assessment of various supplemental carbon (glucose, cellulose) and nitrogen sources.

Main Results:

• Fermentation temperature, duration, and inoculum volume significantly impacted xylanase production (p < 0.001).
• Optimization resulted in a 21.7% increase in xylanase yield.
• Maximum xylanase activity (2,335 U/g) achieved at 45°C for 40 h with specific inoculum volume.
• Supplementation with glucose and cellulose increased relative activity by 10% and 7%, respectively.
• Ammonium salts, nitrates, and organic nitrogen sources significantly reduced xylanase production (p < 0.005).

Conclusions:

• Optimized SSF conditions are critical for maximizing extracellular xylanase production by Thermomyces lanuginosus 195.
• Specific carbon sources can enhance enzyme yield, while certain nitrogen sources are detrimental.
• The findings highlight the potential of T. lanuginosus for efficient xylanase production in SSF.