Related Experiment Video

Updated: Jun 18, 2026

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

Regression of murine lung tumors by the let-7 microRNA

P Trang1, P P Medina, J F Wiggins

  • 1Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.

Oncogene
|December 8, 2009
PubMed

Insights

Loss of let-7 microRNA function promotes lung cancer. Replacing let-7 in mouse models reduced non-small-cell lung cancer tumors, showing therapeutic potential for microRNA replacement therapy.

Area of Science:

  • Molecular biology
  • Genetics
  • Oncology

Background:

  • MicroRNAs (miRNAs) are key regulators of cellular processes.
  • Dysregulation of miRNAs is linked to human diseases, including cancer.

Purpose of the Study:

  • To investigate the role of let-7 microRNA in lung cancer development.
  • To evaluate the therapeutic potential of let-7 replacement in non-small-cell lung cancer (NSCLC).

Main Methods:

  • Assessing the impact of let-7 loss on lung tumor formation in vivo.
  • Administering exogenous let-7 to established NSCLC tumors in mouse models.

Main Results:

  • Loss of let-7 function significantly enhanced lung tumor formation.
  • Exogenous let-7 delivery reduced tumor burden in established NSCLC models.

Conclusions:

  • Let-7 acts as a tumor suppressor in lung cancer.
  • MicroRNA replacement therapy is a promising therapeutic strategy for NSCLC.

More Related Videos

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells07:19

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

The Soft Agar Colony Formation Assay08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...