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Related Experiment Video

Updated: Jun 18, 2026

Quantitative Imaging of Lineage-specific Toll-like Receptor-mediated Signaling in Monocytes and Dendritic Cells from Small Samples of Human Blood

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Effect of aging and oral tolerance on dendritic cell function.

P U Simioni¹, L G R Fernandes, D L Gabriel

¹Departamento de Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas

|December 8, 2009

Summary

Aging does not prevent oral tolerance induction in mice but impairs dendritic cell function. Dendritic cells from older mice show reduced co-stimulatory molecule expression, impacting T cell responses.

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Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

Area of Science:

Immunology
Aging Research
Allergy and Immunology

Background:

Oral tolerance, the immune system's unresponsiveness to ingested antigens, is crucial for preventing allergies.
Dendritic cells (DCs) play a key role in initiating immune responses, including those related to oral tolerance.
The impact of aging on DC function and oral tolerance is not fully understood.

Purpose of the Study:

To investigate how aging affects the induction and maintenance of oral tolerance.
To determine the influence of aging on dendritic cell (DC) function in the context of oral tolerance.
To examine the expression of co-stimulatory molecules on DCs from aged and tolerized mice.

Main Methods:

Oral tolerance was induced in BALB/c mice of various ages (8-80 weeks) using dietary antigens.
Dendritic cells (DCs) were isolated from tolerized, immunized, and control mice.
DC function was assessed by their ability to induce naive T cell proliferation and cytokine secretion (IFN-γ, IL-4, IL-10, TGF-β).
Expression of the CD86 co-stimulatory marker on DCs was quantified.

Main Results:

Oral tolerance was successfully induced in mice of all ages, though antibody levels declined with age.
DCs from young to middle-aged tolerized mice showed reduced capacity to stimulate T cell proliferation and cytokine production compared to immunized mice.
DCs from aged (60-80 weeks) tolerized and immunized mice were similarly ineffective in inducing T cell responses.
A significant reduction in CD86 expression was observed on DCs from both aged and tolerized mice.

Conclusions:

Aging does not impede the establishment of oral tolerance in BALB/c mice.
The aging process significantly impairs dendritic cell function, likely due to decreased CD86 expression.
Reduced DC function in aged mice may compromise their ability to mount effective T cell-mediated immune responses.