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Updated: Jun 18, 2026
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
Published on: September 20, 2016
[FLT3 mutation in patients with acute lymphoblastic leukemia and its clinical significance]
Xin Zhao1, Ke-Jing Tang, Zheng Tian
1State Key Laboratory of Experimental Hematology, Department of Hematologic Oncology, Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.
Objective:
To investigate fms-like tyrosine kinase 3 (FLT3) mutation in patients with acute lymphoblastic leukemia (ALL) and its clinical significance.
Methods:
FLT3 mutation-internal tandem duplication (FLT3-ITD) and FLT3 mutation in the tyrosine kinase domain (FLT3-TKD) were detected using polymerase chain reaction (PCR) in the genomic DNA of 61 ALL patients and 7 healthy volunteers (as reference group). The PCR products of these patients who were detected with FLT3-ITD or FLT3-TKD were sent for sequence analysis.
Results:
In all these 61 ALL patients, FLT3-ITDs were identified in 2 patients and FLT3-TKD were identified in one patient.
Conclusion:
FLT3-ITD and FLT3-TKD exist in a small proportion of ALL patients.
