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Updated: Jun 18, 2026

A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors
Published on: December 7, 2014
Constitutively active Akt contributes to vincristine resistance in human retinoblastoma cells
Hao Zhang1, Bin Li, Shu-wei Bai
1Beijing Institute of Ophthalmology, Beijing Tong Ren Eye Centre, Beijing Tong Ren Hospital, Capital Medical University, Beijing, China.
Abstract:
Akt mediates survival signals which preserve various cancer cells from apoptosis. Its correlation with chemoresistance of cancer cells has attracted much attention. But how Akt affects vincristine-induced cell death in retinoblastoma is unknown. Here we show that Akt activity is involved in vincristine-induced cytotoxicity in retinoblastoma SO-Rb50 cells. Inhibition of Akt can decrease the survival of both sensitive and vincristine-resistant SO-Rb50 cells and increase the sensitivity of both cell lines to vincristine by enhancing the cell apoptosis and increasing the cellular accumulation of the drug. Akt may be a target for reducing drug resistance that improves the treatment for retinoblastoma.
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