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Adapter-directed display: a modular design for shuttling display on phage surfaces.

Kevin Caili Wang¹, Xinwei Wang, Pingyu Zhong

¹Abmaxis Inc., a wholly owned subsidiary of Merck & Co, Inc, WP26-413, 770 Sumneytown Pike, West Point, PA 19486, USA. kevin_wang@merck.com

Journal of Molecular Biology

|December 9, 2009

Summary

A novel adapter-directed phage display system enables modular protein presentation on phage surfaces. This versatile platform allows target proteins to be displayed on various phage coat proteins and formats, enhancing biopaneling applications.

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Area of Science:

Biotechnology
Molecular Biology
Protein Engineering

Background:

Phage display is a powerful technique for selecting proteins with specific binding properties.
Current phage display systems often lack modularity, requiring reengineering for different display formats or target proteins.

Purpose of the Study:

To develop a novel, modular adapter-directed phage display system.
To enable flexible display of target proteins on various phage coat proteins and formats.
To demonstrate cross-species applicability between phage and yeast display systems.

Main Methods:

Constructed a phage display system utilizing specific adapter heterodimerization (GR1 and GR2) for target protein display.
Engineered helper phages to control display valency and formats.

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Demonstrated shuttling display of single-chain Fv antibodies and antigen-binding fragments on different phage coat proteins (pIII, pVII, pVIII).

Applied the adapter-directed display concept to yeast surface display and a cross-species prokaryotic-eukaryotic system.

Main Results:

Successfully developed a modular adapter-directed phage display system.
Achieved flexible display of target proteins on multiple phage coat proteins and formats without reengineering.
Demonstrated successful shuttling display of antibody fragments between multivalent and monovalent formats.
Validated the system's adaptability to yeast surface display and cross-species applications.

Conclusions:

The adapter-directed system offers unprecedented modularity and flexibility in phage display.
This platform facilitates efficient engineering of phage display libraries for diverse applications.
The demonstrated cross-species capability opens new avenues for protein engineering and biopanning.