Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epidermal growth factor-induced cell rounding is sensitive to simulated microgravity.

P J Rijken1, R P de Groot, W Briegleb

  • 1Department of Molecular Cell Biology, University of Utrecht, The Netherlands.

Aviation, Space, and Environmental Medicine
|January 1, 1991
PubMed
Summary

Simulated microgravity enhances epidermal growth factor (EGF)-induced cell rounding in A431 carcinoma cells. This suggests microgravity influences growth factor signaling pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recombinant Newcastle disease viruses with targets for PCR diagnostics for rinderpest and peste des petits ruminants.

Journal of virological methods·2018
Same author

Erratum to: Identification of a restriction point at the M/G1 transition in CHO cells.

Cellular and molecular life sciences : CMLS·2017
Same author

Competition for inorganic substrates among chemoorganotrophic and chemolithotrophic bacteria.

Microbial ecology·2013
Same author

B-50/GAP-43 in neuronal development and repair.

Restorative neurology and neuroscience·2011
Same author

Stable transmission of reversible modifications: maintenance of epigenetic information through the cell cycle.

Cellular and molecular life sciences : CMLS·2010
Same author

Gridded Aclar: preparation methods and use for correlative light and electron microscopy of cell monolayers, by TEM and FIB-SEM.

Journal of microscopy·2010

Area of Science:

  • Cell Biology
  • Gravitational Biology
  • Signal Transduction

Background:

  • Epidermal growth factor (EGF) triggers rapid cell rounding in A431 human epidermoid carcinoma cells.
  • This cellular response is sensitive to temperature and EGF concentration.

Purpose of the Study:

  • To investigate the impact of altered gravity on EGF-induced cell rounding.
  • To determine if microgravity or hypergravity affects EGF signaling in A431 cells.

Main Methods:

  • Experiments utilized a fast-rotating clinostat to simulate microgravity.
  • A centrifuge was employed to simulate hypergravity conditions.
  • EGF-induced cell rounding in A431 cells was observed under these simulated gravity conditions.

Main Results:

Related Experiment Videos

  • Simulated microgravity significantly enhanced EGF-induced cell rounding.
  • Hypergravity conditions did not produce significant effects on this cellular process.
  • These findings indicate a modulation of EGF-induced signal transduction by microgravity.

Conclusions:

  • Simulated microgravity enhances the EGF-induced cell rounding response in A431 cells.
  • Gravity variations, particularly microgravity, play a role in modulating growth factor signaling pathways.
  • Further research into the mechanisms of gravity-dependent signal transduction is warranted.