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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: Jun 18, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

H-DBAS: human-transcriptome database for alternative splicing: update 2010.

Jun-ichi Takeda1, Yutaka Suzuki, Ryuichi Sakate

  • 1Integrated Database and Systems Biology Team, Biomedicinal Information Research Center National Institute of Advanced Industrial Science and Technology, AIST Bio-IT Research Bldg Aomi 2-4-7, Koto-ku, Tokyo 135-0064, Japan.

Nucleic Acids Research
|December 9, 2009
PubMed
Summary

The H-DBAS database now integrates RNA-Seq data to enhance human alternative splicing (AS) annotations. This update identifies protein-coding exons and non-coding AS junctions, improving the analysis of complex AS events.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Alternative splicing (AS) is a key mechanism for generating proteomic diversity in humans.
  • Accurate annotation of AS events is crucial for understanding gene function and regulation.
  • Existing databases may lack comprehensive integration of experimental RNA data for AS annotation.

Purpose of the Study:

  • To enhance the H-DBAS database with RNA-Seq data for improved human alternative splicing (AS) annotation.
  • To identify translationally relevant AS exons and non-coding AS junctions.
  • To provide tools for comparative genomics analysis of AS evolution.

Main Methods:

  • Correlated 148,376,598 RNA-Seq tags with AS exons and splice junctions.
  • Analyzed RNA from cytoplasmic, nuclear, and polysome fractions.
  • Developed a comparative genomics viewer for AS evolution.

Main Results:

  • Identified 90,900 exons likely used for protein synthesis.
  • Discovered 254 human RefSeq transcript-specific AS junctions unique to nuclear RNA, potentially lacking translational impact.
  • Integrated experimental RNA-Seq data with curated full-length cDNA information.

Conclusions:

  • The updated H-DBAS provides a robust platform for analyzing complex human AS.
  • Integration of RNA-Seq data refines the annotation of functional AS events.
  • The new comparative genomics viewer aids in understanding AS evolutionary dynamics.