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Translocation of the yeast dolichol-phosphate-mannose synthase into microsomal membranes
1Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Biochemical and Biophysical Research Communications
|February 14, 1991
Abstract:
Dolichol-phosphate-mannose synthase catalyzes the formation of Dolichol-phosphate-mannose from Dolichol-phosphate and GDP-mannose. Analysis of the primary amino acid sequence of the yeast enzyme predicts a luminal orientation of the enzyme in the endoplasmic reticulum. We analysed the translocation of the Dolichol-phosphate-mannose synthase into dog pancreatic microsomal membranes: resistance to proteolytic attack provides evidence of its luminal orientation and asks for a reevaluation of the topology of the reaction.