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A morphological and phenotypic analysis of Walker 256 cells
H Simpkins1, J M Lehman, J E Mazurkiewicz
1Department of Pathology, Staten Island University Hospital Brooklyn, New York.
Cancer Research
|February 15, 1991
Summary
Walker 256 cells resistant to cisplatin were analyzed. Morphological and phenotypic studies revealed these cells originate from monocytoid, not epithelial, sources, challenging previous assumptions.
Area of Science:
- Cancer Cell Biology
- Hematology
- Oncology
Background:
- Walker 256 cells are utilized in cancer research.
- Understanding the cellular origin of cancer cell lines is crucial for accurate research.
Purpose of the Study:
- To investigate the morphological and phenotypic characteristics of Walker 256 cells.
- To determine the cell of origin for Walker 256 cells, particularly those sensitive and resistant to cisplatin.
Main Methods:
- Morphological analysis using electron microscopy.
- Immunocytochemistry and flow cytometry with monoclonal antibodies.
- Enzyme histochemistry for acid phosphatase activity.
Main Results:
- Two distinct cell populations with differing differentiation were identified.
- Cells lacked epithelial markers but expressed germ and/or hematopoietic cell markers.
- Phenotypic analysis indicated a monocytoid origin, with no evidence of heterogeneity.
- Enzyme histochemistry supported a hematopoietic origin.
Conclusions:
- Walker 256 cells, sensitive and resistant to cisplatin, are of monocytoid origin.
- The findings refute the hypothesis that these cells originate from an epithelial source.
- This research clarifies the cellular lineage of Walker 256 cells for future studies.