Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A sensitive enzyme immunoassay for human basic fibroblast growth factor.

H Watanabe¹, A Hori, M Seno

¹Chemistry Research Laboratory, Takeda Chemical Industries Ltd., Osaka, Japan.

Biochemical and Biophysical Research Communications

|February 28, 1991

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optimisation of Radiation Therapy in Bladder Preservation Therapy for Patients With Clinical Stage T2N0M0 Bladder Cancer.

Clinical oncology (Royal College of Radiologists (Great Britain))·2022

Same author

Cost utility analysis of pharmacist counseling care for breast cancer chemotherapy outpatients.

Die Pharmazie·2019

Same author

Combating 'fake news' and social stigma after the Fukushima Daiichi Nuclear Power Plant incident-the importance of accurate longitudinal clinical data.

QJM : monthly journal of the Association of Physicians·2019

Same author

Death of the sole doctor at Takano Hospital 6 years after the Fukushima nuclear crisis-who is responsible for health care delivery in the Fukushima disaster zone?

QJM : monthly journal of the Association of Physicians·2017

Same author

Mutant botrocetin-2 inhibits von Willebrand factor-induced platelet agglutination.

Journal of thrombosis and haemostasis : JTH·2017

Same author

Structured Frameworks to Increase the Transparency of the Assessment of Benefits and Risks of Medicines: Current Status and Possible Future Directions.

Clinical pharmacology and therapeutics·2015

Same journal

Biosynthesis, characterization and biological potential of microbe-mediated silver nanoparticles using thermophilic actinomycetes, Streptomyces nigra.

Biochemical and biophysical research communications·2026

Same journal

COP9 signalosome 8 mediated autophagy drives proliferation, invasion, and metastasis in pancreatic ductal adenocarcinoma.

Biochemical and biophysical research communications·2026

Same journal

Tumor budding in colorectal cancer: partial EMT, microenvironmental remodeling, and metastatic competence.

Biochemical and biophysical research communications·2026

Same journal

Exploring the therapeutic versatility and multitarget pharmacological potential of acyl hydrazone-hydrazide scaffolds.

Biochemical and biophysical research communications·2026

Same journal

The plasma membrane H<sup>+</sup>-ATPase OSA2 negatively regulates salt tolerance in rice seedlings.

Biochemical and biophysical research communications·2026

Same journal

MiR-425-5p modulation of CREB1 affects inflammatory response and motor recovery after spinal cord injury.

Biochemical and biophysical research communications·2026

See all related articles

A new assay accurately measures human basic fibroblast growth factor (HbFGF) using monoclonal antibodies. This method detected elevated HbFGF in some tumor cells and normal volunteer serum samples.

Area of Science:

Biochemistry
Immunology
Molecular Biology

Background:

Basic fibroblast growth factor (bFGF) plays a crucial role in cell growth, differentiation, and tissue repair.
Dysregulation of bFGF is implicated in various pathological conditions, including cancer.
Sensitive and specific assays are needed to quantify bFGF levels in biological samples.

Purpose of the Study:

To develop and validate a sensitive sandwich enzyme immunoassay for quantifying human basic fibroblast growth factor (HbFGF).
To assess HbFGF production in tumor cell lines and detect its presence in human serum samples.

Main Methods:

Development of a sandwich enzyme immunoassay utilizing three monoclonal antibodies (MAb3H3, MAb98, MAb52).
Conjugation of horseradish peroxidase to the Fab' fragment of MAb3H3, which inhibits HbFGF activity.

Related Experiment Videos

Use of a mixture of MAb52 and MAb98 in the solid phase for capture.

Main Results:

The assay demonstrated high specificity, with no cross-reactivity to human acidic FGF, hst-1/KS3, or acid-denatured HbFGF.
The detection limit of the immunoassay was as low as 1 pg/well.
Elevated HbFGF levels were observed in certain tumor cell lines compared to normal ones.
Immuno-reactive HbFGF was detected in 16 out of 57 normal volunteer serum samples, ranging from 30 to 206 pg/ml.

Conclusions:

The developed sandwich enzyme immunoassay is a sensitive and specific method for quantifying HbFGF.
The assay can differentiate bFGF production levels between tumor and normal cell lines.
HbFGF is detectable in the serum of healthy individuals, suggesting its potential as a biomarker.