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Related Experiment Video

Updated: Jun 17, 2026

Inducing Apical Periodontitis in Mice
10:26

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy.

Andrea M Marcaccini1, Cesar A Meschiari, Leonardo R Zuardi

  • 1Department of Buco-Maxilo-Facial Surgery and Periodontology, Dental School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil.

Journal of Clinical Periodontology
|December 10, 2009
PubMed
Summary

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Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...

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Matrix metalloproteinase-8 (MMP-8), TIMP-2, myeloperoxidase (MPO), and a specific MMP-9 form were elevated in chronic periodontitis patients. These markers significantly decreased after periodontal therapy, indicating treatment effectiveness.

Area of Science:

  • Periodontology
  • Biochemistry
  • Biomarkers

Background:

  • Chronic periodontitis (CP) is a prevalent inflammatory condition affecting periodontal tissues.
  • Gingival crevicular fluid (GCF) contains various enzymes and inhibitors that reflect periodontal health status.
  • Matrix metalloproteinases (MMPs) and their inhibitors (TIMPs) play crucial roles in tissue remodeling and inflammation in periodontal disease.

Purpose of the Study:

  • To compare GCF levels of MMP-8, TIMP-1, TIMP-2, MPO, and MMP-9 between CP patients and controls.
  • To evaluate the changes in these biomarkers 3 months after non-surgical periodontal therapy.

Main Methods:

  • GCF samples were collected from 27 CP patients and 15 controls.
  • MMP-8, MMP-9, TIMP-1, and TIMP-2 were quantified using Enzyme-linked immunoabsorbent assay (ELISA).

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Last Updated: Jun 17, 2026

Inducing Apical Periodontitis in Mice
10:26

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

  • MMP-9 isoforms were analyzed by gelatin zymography, and MPO was measured colorimetrically.

    • Main Results:

    • Baseline GCF showed significantly higher levels of MMP-8, TIMP-2, MPO, and 87 kDa MMP-9 in CP patients compared to controls (p<0.001).
    • These elevated markers decreased significantly (p<0.03) after non-surgical therapy.
    • No significant differences were found in TIMP-1 or total MMP-9 levels between groups at baseline. In controls, TIMP-2 and higher molecular forms of MMP-9 decreased post-therapy (p<0.01).

    Conclusions:

    • Elevated GCF levels of MMP-8, TIMP-2, MPO, and 87 kDa MMP-9 are associated with chronic periodontitis.
    • Non-surgical periodontal therapy leads to a significant reduction in these key inflammatory and tissue-degrading biomarkers.