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High reflection mirrors for pulse compression gratings

S Palmier1, J Neauport, N Baclet

  • 1Commissariat à l'Energie Atomique, Centre d'ETudes Scientifiques et Techniques d'Aquitaine, BP2, F-33114 Le Barp, France. stephanie.palmier@cea.fr

Optics Express
|December 10, 2009
PubMed

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.