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Updated: Jun 17, 2026
Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation
Published on: May 12, 2008
Single-anchor support and supercritical CO2 drying enable high-precision microfabrication of three-dimensional
Shoji Maruo1, Takuya Hasegawa, Naoki Yoshimura
1Department of Mechanical Engineering, Graduate School of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan. maruo@ynu.ac.jp
Abstract:
In high-precision two-photon microfabrication of three-dimensional (3-D) polymeric microstructures, supercritical CO(2) drying was employed to reduce surface tension, which tends to cause the collapse of micro/nano structures. Use of supercritical drying allowed high-aspect ratio microstructures, such as micropillars and cantilevers, to be fabricated. We also propose a single-anchor supporting method to eliminate non-uniform shrinkage of polymeric structures otherwise caused by attachment to the substrate. Use of this method permitted frame models such as lattices to be produced without harmful distortion. The combination of supercritical CO(2) drying and the single-anchor supporting method offers reliable high-precision microfabrication of sophisticated, fragile 3-D micro/nano structures.
