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Subtype identification of the novel A H1N1 and other human influenza A viruses using an oligonucleotide microarray

Xiaoping Kang1, Yongqiang Li, Honghe Sun

  • 1State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Dongdajie Road 20, Fengtai District, 100071 Beijing, China.

Archives of Virology
|December 10, 2009
PubMed

Insights

A new oligonucleotide microarray accurately identifies novel H1N1 influenza viruses, distinguishing them from seasonal strains. This rapid diagnostic tool is crucial for controlling the H1N1 pandemic and other influenza A infections.

Area of Science:

  • Virology
  • Molecular Diagnostics
  • Epidemiology

Background:

  • The 2009 H1N1 influenza A pandemic posed a significant global health threat.
  • Accurate and rapid identification of influenza A subtypes is critical for effective treatment and control strategies.
  • Distinguishing novel H1N1 from seasonal influenza strains is essential for pandemic management.

Purpose of the Study:

  • To develop and validate an oligonucleotide microarray for subtyping human influenza A viruses, including the novel H1N1 strain.
  • To differentiate the novel H1N1 virus from seasonal H1N1 and swine H1N1 influenza viruses.
  • To assess the microarray's performance in identifying clinical cases of novel H1N1.

Main Methods:

  • Development of a multiplex PCR assay targeting hemagglutinin (HA), neuraminidase (NA), and matrix (MP) genes.
  • Design of 59-mer oligonucleotide probes specific for differentiating influenza A subtypes.
  • Application of the oligonucleotide microarray for subtyping reference strains and clinical specimens.

Main Results:

  • The developed microarray accurately identified and subtyped reference strains of human influenza A viruses.
  • The assay successfully distinguished the novel H1N1 strain from seasonal H1N1 and swine H1N1 viruses.
  • Four out of 39 clinical throat swab samples from suspected novel H1N1 cases were correctly identified.

Conclusions:

  • The oligonucleotide microarray is a reliable tool for rapid and accurate subtyping of human influenza A viruses, including the novel H1N1 strain.
  • This diagnostic platform can aid in the surveillance and control of influenza pandemics.
  • The microarray shows potential for use in clinical settings for timely diagnosis of influenza infections.

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