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Related Experiment Video

Updated: Jun 17, 2026

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
08:06

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

Published on: April 22, 2011

Gene expression profiling of the developing mouse kidney and embryo.

Lisa Shaw1, Penny A Johnson, Susan J Kimber

  • 1Faculty of Life Sciences, The University of Manchester, Core Technology Facility, 46 Grafton St, Manchester, M13 9NT, UK. lisa.shaw@manchester.ac.uk

In Vitro Cellular & Developmental Biology. Animal
|December 10, 2009
PubMed
Summary

Identifying novel gene markers for kidney development is crucial. This study found specific genes expressed in developing kidneys and other tissues, aiding in distinguishing renal cells from stem cell populations.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Stem Cell Biology

Background:

  • The metanephros (kidney) forms through reciprocal interactions between the metanephric mesenchyme (MM) and ureteric bud (UB).
  • Identifying specific markers for renal precursor cells is essential for understanding kidney development and for potential therapeutic applications.
  • Previous studies identified renal precursor cell markers but lacked comprehensive analysis in other embryonic tissues.

Purpose of the Study:

  • To identify and characterize gene markers specifically expressed during metanephric (kidney) induction and maturation.
  • To analyze the expression patterns of candidate renal markers in other developing murine embryonic tissues.
  • To identify potential negative markers for kidney development to aid in stem cell differentiation.

Main Methods:

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Last Updated: Jun 17, 2026

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
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Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

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Technique to Target Microinjection to the Developing Xenopus Kidney
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Technique to Target Microinjection to the Developing Xenopus Kidney

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Dissection and Culture of Mouse Embryonic Kidney

Published on: May 17, 2017

  • Assayed expression of up to 19 candidate genes in eight embryonic tissues across five mouse gestation stages (E10.5-E12.5 dpc).
  • Analyzed gene expression patterns to identify markers specific to renal development and maturation.
  • Compared expression profiles in renal tissues with those in other developing organs like the brain and liver.

Main Results:

  • Dcn, Hoxc9, Mest, Wt1, and Ywhaq showed moderate to high expression during metanephric specification and early differentiation.
  • Hoxc9, Ren1, and Wt1 expression characterized mature renal cells.
  • Cd24a, Cdh11, Mest, Scd2, and Sim2 were regulated during brain development; Scd2, Cd24a, and Sip1 were enriched in the developing liver.

Conclusions:

  • Identified novel gene markers with specific expression patterns during kidney development.
  • Several identified markers (e.g., Cd24a, Scd2) are expressed in non-renal tissues, serving as potential negative markers.
  • Combinations of positive and negative markers can improve the isolation and purification of renal cells from heterogeneous stem cell populations.