Related Experiment Video
Updated: Jun 17, 2026
05:08
Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay
Published on: January 31, 2022
How to quantify iron in an aqueous or biological matrix: a technical note
Sébastien Boutry1, Delphine Forge, Carmen Burtea
1Department of General, Organic and Biomedical Chemistry, NMR and Molecular Imaging Laboratory, University of Mons, Mons, Belgium.
Contrast Media & Molecular Imaging
|December 10, 2009
Summary
Quantifying iron in iron oxide nanoparticles is crucial for MRI contrast agents and cell labeling. This study presents convenient methods for accurate iron content measurement in various samples.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Analytical Chemistry
Background:
- Iron oxide nanoparticles are vital as MRI contrast agents and for magnetic cell labeling.
- Accurate quantification of iron content is essential for these applications.
Purpose of the Study:
- To present convenient and quantitative methods for determining iron content.
- To address the need for reliable iron quantification in iron oxide nanoparticle samples.
Main Methods:
- Development and presentation of several convenient analytical methods.
- Application of methods to iron oxide nanoparticle-containing samples.
- Focus on quantitative evaluation of iron concentration.
Main Results:
- Successful presentation of multiple methods for iron quantification.
- Demonstration of the utility of these methods for contrast media and biological samples.
- Establishment of convenient approaches for iron content analysis.
Conclusions:
- The presented methods offer convenient and quantitative solutions for iron analysis.
- Reliable iron quantification is achievable for iron oxide nanoparticle applications.
- These methods support the effective use of iron oxide nanoparticles in diagnostics and research.
Related Concept Videos
01:24Microbes and Other Elemental Cycles
Microbial activity plays a pivotal role in the biogeochemical cycling of iron and manganese, especially at the redox gradients characteristic of stratified aquatic environments. These cycles are driven by microbial transformations between oxidized and reduced forms of the metals, allowing organisms to exploit them for metabolic energy and structural purposes.Iron Cycling Across Redox GradientsIn neutral, oxygen-rich surface waters, iron is predominantly found in its oxidized, insoluble ferric...
00:39Complexometric Titration: Overview
Complexometric titration involves the formation of a complex by reacting a metal ion with one or more ligands. A visual indicator often detects the end point of a complexometric titration. It is added to the metal solution before the titration, forming a stable metal–indicator complex and imparting color to the solution. As the titration approaches the equivalence point, the excess of the added ligand displaces the indicator from the metal–indicator complex, releasing the free indicator. The...
01:18Effects of EDTA on End-Point Detection Methods
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...