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Updated: Jun 17, 2026

Measuring Enzymatic Stability by Isothermal Titration Calorimetry
Published on: March 26, 2019
Esterase activity associated with secretory IgA and free secretory component preparations from human milk
K Kobayashi1, T K Roberts, J P Vaerman
1Department of Experimental Medicine, University of Louvain, Avenue Chapelle-aux-Champs 4 B-1200 Bruxelles, Belgium.
Abstract:
Secretory immunoglobulin A (IgA) and free secretory component were purified from human milk using methods involving (NH4)2SO4 precipitation, ion-exchange and gel-filtration chromatography. It was found that the preparations, although apparently pure by conventional criteria based on immunoelectrophoresis, zone electrophoresis, or ultracentrifugation, were consistently contaminated by two different esterases. These enzymes could apparently not be removed by further ion-exchange chromatography and/or gel filtration. They could, however, be eliminated by passage on an immunoabsorbent made from anti-free secretory component antiserum.
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