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Related Experiment Video

Updated: Jun 17, 2026

Robust Detection of Gene Amplification in Formalin-Fixed Paraffin-Embedded Samples by Fluorescence In Situ Hybridization
03:55

Robust Detection of Gene Amplification in Formalin-Fixed Paraffin-Embedded Samples by Fluorescence In Situ Hybridization

Published on: July 12, 2024

[STR genotyping in unbuffered formalin fixed paraffin embedded tissue].

Yan Liu1, Li Li, Zhen-Min Zhao

  • 1Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, Shanghai, China. qliuyan@163.com

Fa Yi Xue Za Zhi
|December 17, 2009
PubMed
Summary

Forensic DNA analysis using short tandem repeat (STR) genotyping on formalin-fixed paraffin-embedded samples is influenced by storage duration and tissue type. Lung and heart tissues showed better STR profiling success rates over time.

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DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
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DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

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Last Updated: Jun 17, 2026

Robust Detection of Gene Amplification in Formalin-Fixed Paraffin-Embedded Samples by Fluorescence In Situ Hybridization
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Robust Detection of Gene Amplification in Formalin-Fixed Paraffin-Embedded Samples by Fluorescence In Situ Hybridization

Published on: July 12, 2024

Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation
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Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation

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DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
13:32

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

Area of Science:

  • Forensic science
  • Molecular biology
  • Genetics

Context:

  • Formalin-fixed paraffin-embedded (FFPE) tissues are crucial for retrospective forensic investigations.
  • Optimizing DNA extraction and STR genotyping from FFPE samples is essential for reliable identification.
  • Unbuffered formalin fixation presents unique challenges for DNA preservation.

Purpose:

  • To identify key factors affecting short tandem repeat (STR) genotyping success in FFPE samples.
  • To evaluate the impact of storage duration, tissue type, and DNA extraction methods on STR profile quality.
  • To determine the optimal conditions for STR genotyping from archival FFPE specimens.

Summary:

  • STR genotyping success in FFPE samples significantly depends on storage period and tissue type.
  • DNA recovery and STR allele ratios decrease with prolonged sample preservation.
  • Lung and heart tissues demonstrated higher STR genotyping success rates compared to other tissues.

Impact:

  • This research provides critical insights for forensic scientists working with aged FFPE samples.
  • Understanding these factors improves the reliability of DNA profiling in historical cases.
  • The findings aid in developing standardized protocols for DNA analysis from challenging forensic specimens.