Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Single-cell transcriptomics uncovers endothelial progenitor-like remodelling driving human coronary atherosclerosis progression.

Nature cell biology·2026

Same author

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026

Same author

Plasma exosome-derived miRNA-887-5p alleviates high glucose- and lipid-induced endothelial cell dysfunction.

Nutrition & diabetes·2026

Same author

Reliability-aware modality completion with cross-modal distillation for federated learning with missing modalities.

Neural networks : the official journal of the International Neural Network Society·2026

Same author

The Association between Prenatal Quaternary Ammonium Compounds (QACs) Exposure and Neonatal Birth Obesity: Insights from Epidemiology and <i>In Vitro</i> Evidence.

Environment & health (Washington, D.C.)·2026

Same author

Association Between HIV Infection Duration and Left Ventricular Concentric Remodeling and Myocardial Fibrosis: A Cross-Sectional Study Using Cardiac MRI.

Journal of magnetic resonance imaging : JMRI·2026

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Sensitive bifunctional aptamer-based electrochemical biosensor for small molecules and protein.

Chunyan Deng¹, Jinhua Chen, Lihua Nie

¹State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.

Analytical Chemistry

|December 17, 2009

Summary

A novel bifunctional electrochemical biosensor was developed for sensitive detection of adenosine and lysozyme. This aptasensor utilizes aptamer immobilization and DNA-functionalized gold nanoparticles for enhanced performance.

More Related Videos

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Related Experiment Videos

Last Updated: Jun 17, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Area of Science:

Electrochemistry
Biosensors
Nanotechnology

Background:

Developing sensitive and selective biosensors is crucial for disease diagnosis and monitoring.
Bifunctional biosensors capable of detecting multiple analytes offer advantages in efficiency and sample volume reduction.

Purpose of the Study:

To develop a bifunctional electrochemical biosensor for the simultaneous or individual detection of adenosine and lysozyme.
To enhance the sensitivity and performance of the biosensor through aptamer design and nanomaterial integration.

Main Methods:

Immobilization of dual aptamer units for adenosine and lysozyme onto a gold electrode via DNA/DNA duplex formation.
Utilizing the structural switching of aptamers upon target binding to alter interfacial properties.
Employing DNA-functionalized gold nanoparticles (DNA-AuNPs) for signal amplification.
Characterizing interfacial changes using cyclic voltammetry (CV) with [Ru(NH3)6](3+) redox probe.

Main Results:

The biosensor demonstrated sensitive detection of both adenosine (0.02 nM) and lysozyme (0.01 µg/mL).
The DNA-AuNPs amplification strategy significantly enhanced the sensor's sensitivity by increasing the loading of the redox probe.
The aptasensor exhibited high sensitivity and bifunctional recognition capabilities.

Conclusions:

A simple and generalizable model for a bifunctional aptasensor was successfully established.
The developed electrochemical biosensor offers a promising platform for sensitive and selective detection of small molecules and proteins.
The integration of aptamers and DNA-AuNPs provides a robust approach for advanced biosensing applications.