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User-loaded SlipChip for equipment-free multiplexed nanoliter-scale experiments.

Liang Li¹, Wenbin Du, Rustem Ismagilov

¹Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, USA.

Journal of the American Chemical Society

|December 17, 2009

Summary

A novel SlipChip enables multiplexed nanoliter experiments, optimizing protein crystallization screening with minimal sample. This microfluidic approach successfully identified crystallization conditions and yielded higher-resolution protein structures.

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Area of Science:

Biochemistry
Microfluidics
Structural Biology

Background:

Multiplexed nanoliter-scale experiments require precise fluid handling.
Screening crystallization conditions traditionally demands significant sample volumes and resources.

Purpose of the Study:

To develop and validate a user-loaded, equipment-free SlipChip for multiplexed nanoliter-scale experiments.
To optimize protein crystallization screening and structure determination using microfluidics.

Main Methods:

A SlipChip design was employed for controlled mixing of samples with reagents at various ratios.
Protein crystallization screening was performed using approximately 10 microL of glutaryl-CoA dehydrogenase.
Crystallization conditions were identified and scaled up, with crystals analyzed by X-ray diffraction.

Main Results:

The SlipChip successfully screened 528 conditions for glutaryl-CoA dehydrogenase crystallization.
Identified conditions led to successful crystallization and higher-resolution protein structure determination.
The SlipChip demonstrated reliable fluid handling across diverse physicochemical properties.

Conclusions:

The user-loaded SlipChip is a versatile platform for nanoliter-scale experiments, particularly protein crystallization.
This microfluidic approach reduces sample consumption and enhances structural biology studies.
The technology holds promise for applications in enzyme kinetics, cell-based assays, and chemical reactions.