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Updated: Jun 17, 2026
05:48
Rapid Generation of Amyloid from Native Proteins In vitro
Published on: December 5, 2013
Amyloidogenicity and aggregate cytotoxicity of human glucagon-like peptide-1 (hGLP-1)
S Poon1, N R Birkett, S B Fowler
1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK.
Protein and Peptide Letters
|December 17, 2009
Abstract:
The potential of human glucagon-like peptide-1 (hGLP-1) as a therapeutic agent is limited by its high aggregation propensity. We show that hGLP-1 forms amyloid-like structures that are preceded by cytotoxic aggregates, suggesting that aggregation of biopharmaceuticals could present a cytotoxic risk to patients besides the reported increased risk in immunogenicity.
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Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining, normally used to...
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