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Updated: Jun 17, 2026

Detection and Isolation of Campylobacter spp. from Raw Meat

Detection and Isolation of Campylobacter spp. from Raw Meat

Published on: February 23, 2024

Method for bacteriophage isolation against target Campylobacter strains.

C Carvalho¹, M Susano, E Fernandes

¹IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

Letters in Applied Microbiology

|December 17, 2009

Summary

This summary is machine-generated.

Researchers isolated Campylobacter coli bacteriophages (phages) using a targeted enrichment method. This technique efficiently identifies phages effective against specific bacterial strains, aiding in controlling foodborne illnesses and antibiotic-resistant bacteria.

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Area of Science:

Food Microbiology
Bacteriophage Research
Antimicrobial Resistance

Background:

Poultry meat is a significant source of Campylobacter, a bacterium causing foodborne illnesses.
Campylobacter infections pose a public health challenge, exacerbated by rising antimicrobial resistance.

Purpose of the Study:

To isolate lytic bacteriophages (phages) specifically targeting Campylobacter coli strains.
To develop an efficient method for isolating phages active against specific bacterial pathogens.

Main Methods:

Enrichment of chicken samples in broth with target C. coli strains and salts (CaCl2, MgSO4).
Isolation and characterization of bacteriophages active against C. coli and C. jejuni.

Main Results:

Isolated 43 phages active against 83% of target C. coli strains.
65% of isolated phages demonstrated activity against Campylobacter jejuni strains.

Conclusions:

Targeted pathogen enrichment significantly enhances phage isolation efficiency for specific strains.
This method is valuable for phage therapy, rapidly identifying phages against clinical or foodborne isolates.
Accelerates the search for effective phages, crucial for combating multi-drug resistant bacteria.