Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Transport into the Inner Mitochondrial Membrane

Protein Transport into the Inner Mitochondrial Membrane

Nuclear encoded mitochondrial precursors are imported to the inner membrane in a multistep process involving two separate translocons, TIM22 and TIM23. TIM23 is a cation-selective pore that remains closed by the N terminal segment of the protein. Negative charges on the TIM23 act as a receptor for the incoming precursor, pulling the positively charged matrix-targeting sequence for peptide insertion and translocation.
Transport of mitochondrial precursors across the TIM23 channel is driven by...

Translocation of Proteins into the Mitochondria

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Allosteric modulation of the catalytic VYD loop in Slingshot by its N-terminal domain underlies both Slingshot auto-inhibition and activation.

The Journal of biological chemistry·2018

Same author

Isolated unilateral pulmonary vein atresia with hemoptysis in a child: A case report and literature review.

Medicine·2018

Same author

Clinical factors associated with intestinal strangulating obstruction and recurrence in adhesive small bowel obstruction: A retrospective study of 288 cases.

Medicine·2018

Same author

[Determination of organic acids in 1, 2-butylene oxide products by valve switch-ion chromatography].

Se pu = Chinese journal of chromatography·2018

Same author

Disruption of Planar Cell Polarity Pathway Attributable to Valproic Acid-Induced Congenital Heart Disease through Hdac3 Participation in Mice.

Chinese medical journal·2018

Same author

Multispectral and large bandwidth achromatic imaging with a single diffractive photon sieve.

Optics express·2018

Same journal

Characterization of putative proteins encoded by variable ORFs in white spot syndrome virus genome.

BMC structural biology·2019

Same journal

Correction to: Classification of the human THAP protein family identifies an evolutionarily conserved coiled coil region.

BMC structural biology·2019

Same journal

Effect of low complexity regions within the PvMSP3α block II on the tertiary structure of the protein and implications to immune escape mechanisms.

BMC structural biology·2019

Same journal

QRNAS: software tool for refinement of nucleic acid structures.

BMC structural biology·2019

Same journal

Classification of the human THAP protein family identifies an evolutionarily conserved coiled coil region.

BMC structural biology·2019

Same journal

A new technique for predicting intrinsically disordered regions based on average distance map constructed with inter-residue average distance statistics.

BMC structural biology·2019

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry

Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry

Published on: January 12, 2024

TIM-Finder: a new method for identifying TIM-barrel proteins.

Jing-Na Si¹, Ren-Xiang Yan, Chuan Wang

¹State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. sijingna@gmail.com

BMC Structural Biology

|December 17, 2009

Summary

This summary is machine-generated.

A new computational tool, TIM-Finder, enhances the identification of triosephosphate isomerase (TIM)-barrel proteins. This method improves proteome-wide analysis and accelerates protein discovery.

More Related Videos

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Published on: December 12, 2025

Related Experiment Videos

Last Updated: Jun 17, 2026

Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry

Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry

Published on: January 12, 2024

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Published on: December 12, 2025

Area of Science:

Bioinformatics
Computational Biology
Structural Biology

Background:

The triosephosphate isomerase (TIM)-barrel fold is prevalent across organisms, with diverse protein functions.
Sensitive detection of TIM-barrel proteins is crucial for exploring their sequence-structure landscape.

Purpose of the Study:

To develop an advanced computational tool for sensitive and accurate identification of TIM-barrel proteins.
To improve upon existing methods for TIM-barrel protein detection in proteomic datasets.

Main Methods:

Integration of three descriptors: sequence-alignment (PSI-BLAST e-values/bit scores), secondary structure element alignment (SSEA), and PROSITE functional motifs.
Application of Support Vector Machine (SVM) for descriptor combination and model training.
Validation using the complete proteome of Bacillus subtilis.

Main Results:

The developed method, TIM-Finder, demonstrates superior performance in TIM-barrel protein identification.
TIM-Finder identified 194 TIM-barrel proteins in Bacillus subtilis at a 99% confidence level.
Outperformed standard PSI-BLAST searches and existing fold recognition methods.

Conclusions:

TIM-Finder is a competitive and effective tool for proteome-wide TIM-barrel protein identification.
The TIM-Finder web server is publicly accessible for research use.