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Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis
Published on: June 14, 2019
Sensitive HPV detection in oropharyngeal cancers.
David M Winder1, Siolian L R Ball, Katie Vaughan
1Dept of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK. dmw24@cam.ac.uk
BMC Cancer
|December 17, 2009
Summary
The PGMY09/11 primer set is most effective for detecting human papillomaviruses (HPV) in various clinical samples, including oropharyngeal cancers. A nested PCR approach may be necessary for low-level HPV detection.
Area of Science:
- Oncology
- Virology
- Molecular Biology
Background:
- Human papillomaviruses (HPV) are linked to various benign and malignant tumors, notably cervical cancer.
- Rising incidence of ano-genital warts, HPV-anal cancer, and oropharyngeal cancers necessitates improved detection methods.
- Accurate HPV detection is crucial for diagnosing and managing HPV-related cancers.
Purpose of the Study:
- To directly compare the efficacy of three common primer sets (PGMY09/11, MY09/11, GP5+/6+) for HPV detection in diverse clinical samples.
- To evaluate an optimized PCR protocol for detecting HPV in oropharyngeal cancer samples.
- To establish a reliable PCR detection protocol for HPV in clinical settings.
Main Methods:
- PCR amplification using PGMY09/11, MY09/11, and GP5+/6+ primer sets on genital warts, cervical brushings, and vulval biopsies.
- Negative samples re-tested with PGMY/GP nested PCR and amplicons sequenced for confirmation and typing.
- Optimized PCR protocol compared against a line blot assay for oropharyngeal cancer samples.
Main Results:
- PGMY09/11 primers showed higher HPV detection rates in cervical brushings (100%) and genital warts (92.9%) compared to MY09/11 and GP5+/6+.
- HPV detection rates in vulval biopsies varied: MY09/11 (63.6%), GP5+/6+ (54.5%), and PGMY09/11 (54.5%).
- A PGMY-GP nested PCR protocol detected HPV in 66.7% of oropharyngeal cancer samples.
Conclusions:
- PGMY09/11 primers are recommended for primary PCR screening of HPV in clinical samples due to superior detection rates.
- MY09/11 and GP5+/6+ primers are less sensitive but may be suitable for specific applications like cervical samples.
- A nested PCR approach, such as the PGMY-GP system, is valuable for confirming negativity and detecting low-level HPV infections, particularly in challenging samples like those from oropharyngeal cancers.
