Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bacterial Transformation

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...

Bacterial Transformation

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...

Transformation

Transformation

Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...

Transduction

Transduction

Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ex vivo assay for organ-specific cancer cell invasion.

Journal of biological engineering·2026

Same author

Genomic and phylogenomic analyses reveal extensive diversity among soil-derived Acinetobacter baumannii isolates from Nigeria.

Scientific reports·2026

Same author

Beyond Hematology-Current Insights into Chimeric Antigen Receptor (CAR) T-Cell Therapy for Skin and Connective Tissue Disorders.

Cells·2026

Same author

Retrospective Evaluation of the Impact of the COVID-19 Pandemic on the Incidence of Alopecia Areata in a Single Dermatological Department.

Journal of clinical medicine·2026

Same author

Multimodal Assessment of Kidney Quality During 24-h of Normothermic Machine Perfusion.

Artificial organs·2026

Same author

Acinetobacter baumannii's lifestyle includes soil-dwelling colonization of decaying plant material and airborne spread.

Nature communications·2026

Search research articles

Related Experiment Video

Updated: Jun 17, 2026

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

Published on: October 8, 2013

A simple and rapid method of bacterial transformation.

Gottfried Wilharm¹, Daniela Lepka, Franziska Faber

¹Robert Koch-Institute, Wernigerode Branch, Burgstr. 37, D-38855 Wernigerode, Germany. wilharmg@rki.de

Journal of Microbiological Methods

|December 17, 2009

Summary

A new method uses nanofibers to transform bacteria, making genetic modification easier. This technique successfully transformed common bacteria like E. coli, offering a user-friendly approach for researchers.

More Related Videos

Transformation of Plasmid DNA into E. coli Using the Heat Shock Method

Transformation of Plasmid DNA into E. coli Using the Heat Shock Method

Published on: August 1, 2007

Related Experiment Videos

Last Updated: Jun 17, 2026

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae

Published on: October 8, 2013

Transformation of Plasmid DNA into E. coli Using the Heat Shock Method

Transformation of Plasmid DNA into E. coli Using the Heat Shock Method

Published on: August 1, 2007

Area of Science:

Microbiology
Molecular Biology
Biotechnology

Background:

Bacterial transformation is crucial for genetic research and biotechnology.
Existing transformation methods can be complex and time-consuming.

Purpose of the Study:

To verify and establish a user-friendly protocol for bacterial transformation using nanofibers.
To demonstrate the efficacy of nanofiber-mediated DNA delivery in various bacterial species.

Main Methods:

Utilizing sepiolite nanofibers as a non-toxic, inexpensive delivery vehicle for transforming DNA.
Mixing bacteria with a buffered sepiolite suspension and transforming DNA.
Spreading the mixture directly onto selective agar plates for selection.

Main Results:

Successfully transformed three distinct bacterial species: Escherichia coli, Yersinia enterocolitica, and Acinetobacter baumannii.
Verified the principle of nanofiber-mediated bacterial transformation.
Established a straightforward and efficient protocol.

Conclusions:

Nanofiber-mediated bacterial transformation is a viable and effective method.
The developed protocol is user-friendly and applicable to diverse bacterial species.
Sepiolite nanofibers offer a cost-effective and safe alternative for DNA delivery in bacteria.