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Updated: Jun 17, 2026

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Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Published on: August 15, 2019
In vitro detection and primary cultivation of bacteria producing materials inhibitory to ruminal methanogens
Rosalind A Gilbert1, Diane Ouwerkerk, Li Hua Zhang
1Animal Research Institute, Queensland Primary Industries and Fisheries, Yeerongpilly, Queensland, 4105, Australia. ros.gilbert@deedi.qld.gov.au
Journal of Microbiological Methods
|December 17, 2009
Abstract:
A novel method for screening bacterial isolates for their potential to inhibit the growth of ruminal methanogenic Archaea was developed using a modification of the soft agar overlay technique, formally used for the isolation of lytic bacteriophages. This method may be used in the specific, hydrogen-rich conditions required for the growth of ruminal methanogenic Archaea.

