Related Experiment Video
Updated: Jun 17, 2026
Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
Published on: October 30, 2013
Gene profiling of cyclosporin-enhanced transitional cell carcinoma in rat model
1Kidney Transplantation Program, Department of Urology, Beijing Chaoyang Hospital of Capital Medical University, 8 Gong Ti Dong Lu, Chaoyang District, Beijing 100020, China. zxd581@263.net
Objective:
We sought to study the effect of cyclosporine (CsA) on development of malignancy.
Materials And Methods:
The observation was performed in a rat model, in which transitional cell carcinoma of urinary bladder was induced with N-butyl-N-(-4-hydroxybutyl) nitrosamine. CsA was added in the food for the rats. At the end of 30 weeks, we examined the tumor burden in the urinary bladders, and compared gene expressions between the CsA-enhanced and non-CsA-enhanced tumor groups by gene profiling.
Results:
CsA feeding increased tumor burden: 2.3 +/- 0.9 versus 1.1 +/- 0.5 g (P < .05). Gene profiling showed many variations involved in CsA enhanced malignant development. Twenty-three genes with known functions were upregulated, and 46 genes with known functions downregulated. In all, 111 genes were involved in the CsA-enhanced malignant development. The regulated genes in the present study constituted 23 pathways mostly involved in carcinogenesis.
Conclusions:
CsA plays an important role in tumor development through gene regulation, which may constitute pathways to malignant progression.
