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ESBL-producing E. coli in Austrian sewage sludge.

Franz Ferdinand Reinthaler1, Gebhard Feierl, Herbert Galler

  • 1Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Universitätsplatz 4, 8010 Graz, Austria. franz.reinthaler@medunigraz.at

Water Research
|December 17, 2009
PubMed
Summary
This summary is machine-generated.

Sewage sludge frequently contains ESBL-producing E. coli. Thermal treatment and lime stabilization effectively reduce these pathogens, while other methods may increase contamination.

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Area of Science:

  • Environmental microbiology
  • Public health

Background:

  • Sewage sludge can harbor antibiotic-resistant bacteria.
  • Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) pose a significant public health risk.

Purpose of the Study:

  • To determine the prevalence of ESBL-producing E. coli in sewage sludge.
  • To evaluate the efficacy of various sewage sludge treatment methods in reducing bacterial contamination.

Main Methods:

  • Monthly sampling of sewage sludge from five treatment plants over nine months.
  • Testing for ESBL E. coli presence and quantifying colony-forming units (CFU) of E. coli and coliforms.
  • Assessing the impact of aerobic/anaerobic digestion, lime stabilization, and thermal treatment.

Main Results:

  • ESBL-producing E. coli were detected in 61.1% of 72 sewage sludge samples.
  • CTX-M and TEM bla genes were identified in ESBL-producing strains.
  • Thermal treatment and lime stabilization, post-dehydration, significantly reduced E. coli and coliform counts.
  • Stabilization and dehydration alone led to an increase in bacterial populations, including ESBL E. coli.

Conclusions:

  • Sewage sludge is a reservoir for ESBL-producing E. coli.
  • Effective sludge treatment is crucial for mitigating the spread of antibiotic resistance.
  • Thermal treatment and lime stabilization are effective methods for reducing pathogen load in sewage sludge.