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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Fibril-associated Collagen01:11

Fibril-associated Collagen

Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...

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Multiple bovine FcgammaRIIb sub-isoforms generated by alternative splicing.

Matthew A Firth1, Kuldeep S Chattha1, Douglas C Hodgins1

  • 1Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada N1G 2W1.

Veterinary Immunology and Immunopathology
|December 17, 2009
PubMed
Summary

Cattle possess multiple FcgammaRIIb (CD32) receptor isoforms, similar to humans and mice. This discovery enhances our understanding of bovine immune responses and immunoglobulin regulation.

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Area of Science:

  • Immunology
  • Veterinary Science
  • Molecular Biology

Background:

  • Fc receptors (FcR) link antibodies to cellular functions but are understudied in livestock.
  • FcgammaRII (CD32) has known activating and inhibitory isoforms in humans and mice.
  • Previous research identified only one FcgammaRIIb isoform in cattle, unlike other species.

Purpose of the Study:

  • To characterize the expression profile of FcgammaRIIb in bovine blood mononuclear cells.
  • To identify novel FcgammaRIIb transcripts in cattle.
  • To compare bovine FcgammaRIIb isoforms with those in humans and mice.

Main Methods:

  • Collected blood samples from dairy and beef cattle breeds.
  • Utilized RT-PCR to analyze FcgammaRIIb mRNA expression.
  • Sequenced and analyzed novel bovine FcgammaRIIb transcripts.

Main Results:

  • Identified two previously uncharacterized bovine FcgammaRIIb transcripts, in addition to the known one.
  • One novel transcript showed high homology to human/murine FcgammaRIIb1 and was widely expressed.
  • The second novel transcript was homologous to murine FcgammaRIIb3 and had a limited expression profile.

Conclusions:

  • Cattle express multiple FcgammaRIIb sub-isoforms, mirroring findings in humans and mice.
  • These findings add complexity to IgG-mediated immunity regulation in cattle.
  • Provides new insights into Fc receptor roles in bovine antigen acquisition and presentation.